Site-selective artificial ribonuclease using pinpoint RNA activation
نویسندگان
چکیده
منابع مشابه
Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease
Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulges (up to 4 nt) were readily formed upon oligonucleotide hybridization, wherea...
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Recent progress regarding the development of artificial site-selective DNA cutters by chemical approaches are reviewed herein, with a special focus on site-selective cutters for single-stranded DNA. We employ a Ce(IV)/EDTA complex to serve as the catalyst, because it efficiently and selectively cuts single-stranded DNA. Using two complementary oligonucleotide additives, a gap structure is forme...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2001
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/1.1.131